口腔科

订阅

MedSci主页 / 所有栏目 / 口腔科

J Endod：骨髓移植术后骨髓来源细胞迁移至牙髓需要的时间

2018-01-11 lishiting MedSci原创

BMT骨髓来源细胞
关注

这篇研究是通过骨髓移植术(BMT)的方法评估绿色荧光蛋白表达阳性转基因小鼠(GFP+小鼠)的骨髓来源细胞(BMDCs)迁移至GFP受体阴性野生型小鼠(GFP-小鼠)的牙髓中所需时间，并将其作为追踪GFP+小鼠BMDCs (GFP+ BMDCs)的体内模型。

这篇研究是通过骨髓移植术(BMT)的方法评估绿色荧光蛋白表达阳性转基因小鼠(GFP+小鼠)的骨髓来源细胞(BMDCs)迁移至GFP受体阴性野生型小鼠(GFP-小鼠)的牙髓中所需时间，并将其作为追踪GFP+小鼠BMDCs (GFP+ BMDCs)的体内模型。研究将GFP+ BMDCs注射入被辐照过的GFP-小鼠体内。随后，在BMT后24小时， 48小时， 4， 7和14天以及7周时分离GFP-小鼠的上颌弓、胫骨和股骨并进行处理。使用激光共聚焦显微镜分析评估牙髓中GFP+ BMDCs的存在情况，并通过BM流式细胞仪评估GFP+ BMDCs移植物的功效。激光共聚焦显微镜分析显示，在BMT后14天至7周间，GFP-小鼠的牙髓中存在GFP+ BMDCs。而在术后24小时， 48小时， 4和7天间未检测到GFP+ BMDCs的存在。BM流式细胞仪对GFP-小鼠的分析显示，在BMT后24小时， 48小时和4天，GFP+ BMDCs的数量不断增加，在7天至7周时稳定。结论：这篇研究表明，在BMT后14天至7周，牙髓内存在GFP+ BMDCs。另外，使用GFP+动物和BMT的方法作为追踪GFP+ BMD

版权声明：
本网站所有内容来源注明为“梅斯医学”或“MedSci原创”的文字、图片和音视频资料，版权均属于梅斯医学所有。非经授权，任何媒体、网站或个人不得转载，授权转载时须注明来源为“梅斯医学”。其它来源的文章系转载文章，或“梅斯号”自媒体发布的文章，仅系出于传递更多信息之目的，本站仅负责审核内容合规，其内容不代表本站立场，本站不负责内容的准确性和版权。如果存在侵权、或不希望被转载的媒体或个人可与我们联系，我们将立即进行删除处理。
在此留言
评论区 (5)
#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=1640931, encodeId=f77816409317a, content=<a href='/topic/show?id=7c99939e3fd' target=_blank style='color:#2F92EE;'>#迁移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=93973, encryptionId=7c99939e3fd, topicName=迁移)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=hb2008ye, createdTime=Tue Oct 09 10:32:00 CST 2018, time=2018-10-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1998934, encodeId=3d13199893431, content=<a href='/topic/show?id=8e2ee5200ee' target=_blank style='color:#2F92EE;'>#移植术#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=75200, encryptionId=8e2ee5200ee, topicName=移植术)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=63a4277, createdName=cy0324, createdTime=Mon Jun 04 04:32:00 CST 2018, time=2018-06-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1669632, encodeId=3cb816696320a, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Fri Feb 09 09:32:00 CST 2018, time=2018-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1529583, encodeId=9cab15295838b, content=<a href='/topic/show?id=6264e80409d' target=_blank style='color:#2F92EE;'>#细胞迁移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78040, encryptionId=6264e80409d, topicName=细胞迁移)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7d5e11955593, createdName=amy0554, createdTime=Sat Jan 13 11:32:00 CST 2018, time=2018-01-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=277598, encodeId=9a132e759878, content=^_^^_^^_^^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Thu Jan 11 20:44:23 CST 2018, time=2018-01-11, status=1, ipAttribution=)]
    2018-10-09 hb2008ye

    #迁移#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1640931, encodeId=f77816409317a, content=<a href='/topic/show?id=7c99939e3fd' target=_blank style='color:#2F92EE;'>#迁移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=93973, encryptionId=7c99939e3fd, topicName=迁移)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=hb2008ye, createdTime=Tue Oct 09 10:32:00 CST 2018, time=2018-10-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1998934, encodeId=3d13199893431, content=<a href='/topic/show?id=8e2ee5200ee' target=_blank style='color:#2F92EE;'>#移植术#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=75200, encryptionId=8e2ee5200ee, topicName=移植术)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=63a4277, createdName=cy0324, createdTime=Mon Jun 04 04:32:00 CST 2018, time=2018-06-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1669632, encodeId=3cb816696320a, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Fri Feb 09 09:32:00 CST 2018, time=2018-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1529583, encodeId=9cab15295838b, content=<a href='/topic/show?id=6264e80409d' target=_blank style='color:#2F92EE;'>#细胞迁移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78040, encryptionId=6264e80409d, topicName=细胞迁移)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7d5e11955593, createdName=amy0554, createdTime=Sat Jan 13 11:32:00 CST 2018, time=2018-01-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=277598, encodeId=9a132e759878, content=^_^^_^^_^^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Thu Jan 11 20:44:23 CST 2018, time=2018-01-11, status=1, ipAttribution=)]
    2018-06-04 cy0324

    #移植术#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1640931, encodeId=f77816409317a, content=<a href='/topic/show?id=7c99939e3fd' target=_blank style='color:#2F92EE;'>#迁移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=93973, encryptionId=7c99939e3fd, topicName=迁移)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=hb2008ye, createdTime=Tue Oct 09 10:32:00 CST 2018, time=2018-10-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1998934, encodeId=3d13199893431, content=<a href='/topic/show?id=8e2ee5200ee' target=_blank style='color:#2F92EE;'>#移植术#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=75200, encryptionId=8e2ee5200ee, topicName=移植术)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=63a4277, createdName=cy0324, createdTime=Mon Jun 04 04:32:00 CST 2018, time=2018-06-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1669632, encodeId=3cb816696320a, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Fri Feb 09 09:32:00 CST 2018, time=2018-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1529583, encodeId=9cab15295838b, content=<a href='/topic/show?id=6264e80409d' target=_blank style='color:#2F92EE;'>#细胞迁移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78040, encryptionId=6264e80409d, topicName=细胞迁移)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7d5e11955593, createdName=amy0554, createdTime=Sat Jan 13 11:32:00 CST 2018, time=2018-01-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=277598, encodeId=9a132e759878, content=^_^^_^^_^^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Thu Jan 11 20:44:23 CST 2018, time=2018-01-11, status=1, ipAttribution=)]
    2018-02-09 jxrzshh

    #牙髓#

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1640931, encodeId=f77816409317a, content=<a href='/topic/show?id=7c99939e3fd' target=_blank style='color:#2F92EE;'>#迁移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=93973, encryptionId=7c99939e3fd, topicName=迁移)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=hb2008ye, createdTime=Tue Oct 09 10:32:00 CST 2018, time=2018-10-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1998934, encodeId=3d13199893431, content=<a href='/topic/show?id=8e2ee5200ee' target=_blank style='color:#2F92EE;'>#移植术#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=75200, encryptionId=8e2ee5200ee, topicName=移植术)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=63a4277, createdName=cy0324, createdTime=Mon Jun 04 04:32:00 CST 2018, time=2018-06-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1669632, encodeId=3cb816696320a, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Fri Feb 09 09:32:00 CST 2018, time=2018-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1529583, encodeId=9cab15295838b, content=<a href='/topic/show?id=6264e80409d' target=_blank style='color:#2F92EE;'>#细胞迁移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78040, encryptionId=6264e80409d, topicName=细胞迁移)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7d5e11955593, createdName=amy0554, createdTime=Sat Jan 13 11:32:00 CST 2018, time=2018-01-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=277598, encodeId=9a132e759878, content=^_^^_^^_^^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Thu Jan 11 20:44:23 CST 2018, time=2018-01-11, status=1, ipAttribution=)]
    2018-01-13 amy0554

    #细胞迁移#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1640931, encodeId=f77816409317a, content=<a href='/topic/show?id=7c99939e3fd' target=_blank style='color:#2F92EE;'>#迁移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=93973, encryptionId=7c99939e3fd, topicName=迁移)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=hb2008ye, createdTime=Tue Oct 09 10:32:00 CST 2018, time=2018-10-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1998934, encodeId=3d13199893431, content=<a href='/topic/show?id=8e2ee5200ee' target=_blank style='color:#2F92EE;'>#移植术#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=75200, encryptionId=8e2ee5200ee, topicName=移植术)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=63a4277, createdName=cy0324, createdTime=Mon Jun 04 04:32:00 CST 2018, time=2018-06-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1669632, encodeId=3cb816696320a, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Fri Feb 09 09:32:00 CST 2018, time=2018-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1529583, encodeId=9cab15295838b, content=<a href='/topic/show?id=6264e80409d' target=_blank style='color:#2F92EE;'>#细胞迁移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78040, encryptionId=6264e80409d, topicName=细胞迁移)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7d5e11955593, createdName=amy0554, createdTime=Sat Jan 13 11:32:00 CST 2018, time=2018-01-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=277598, encodeId=9a132e759878, content=^_^^_^^_^^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Thu Jan 11 20:44:23 CST 2018, time=2018-01-11, status=1, ipAttribution=)]
    2018-01-11 wqkm

    ^_^^_^^_^^_^^_^

    0