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J Endod：牙髓病用材料在临床储存前后的细菌污染分析

2017-09-29 lishiting MedSci原创

牙科耗材临床存储细菌携带
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这篇研究的目的是为了评估牙髓病科耗材(牙胶尖、橡皮障、纸制混合垫、填塞剂和牙科用海绵[EISs])在临床使用和储存前后的细菌污染情况。

这篇研究的目的是为了评估牙髓病科耗材(牙胶尖、橡皮障、纸制混合垫、填塞剂和牙科用海绵[EISs])在临床使用和储存前后的细菌污染情况。在临床使用期间，研究将牙科用材料按照3个时间节点随机分为3份(t0， 开包时； t1， 7天时； t2， 14天时)。向牙胶尖和填塞剂(25 mg)滴加1 mL 磷酸盐缓冲液(PBS)。向橡皮障、纸制混合垫和EIS中添加25 mL PBS。在混悬液涡旋、离心及去除上清液后，沉淀物再次悬浮于1 mL PBS内，并放置于苛养厌氧菌琼脂上进行需要和厌氧孵育。通过基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)鉴定生长克隆情况。提取每个样本800 μL溶液的DNA后，通过定量聚合酶链式反应检测细菌接种总量。MALDI-TOF MS检测结果显示，所有受检材料在3个时间节点均有不同数量的样本污染(除了t0时的EIS)。分离出来最多的细菌总属为丙酸杆菌属(42%)和葡萄球菌属(32%)。通过依赖于非细菌培养的实验方法，所有受试材料在3个时间节点(除了t0时的牙胶尖及t0， t1和t2时的填塞剂)均携带细菌DNA。结论：大多数受试材料的样本在临床储存前后均

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    2018-05-02 jxrzshh

    #牙髓#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1669604, encodeId=3072166960482, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Wed May 02 21:06:00 CST 2018, time=2018-05-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1953612, encodeId=ee681953612d6, content=<a href='/topic/show?id=86156e68526' target=_blank style='color:#2F92EE;'>#牙髓病#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67685, encryptionId=86156e68526, topicName=牙髓病)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e093393, createdName=luwei00, createdTime=Wed Feb 28 10:06:00 CST 2018, time=2018-02-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=249839, encodeId=dd552498392c, content=学习了.担忧, beContent=null, objectType=article, channel=null, level=null, likeNumber=40, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzbCrNlTIlTfsCoY1L9dvM1PibZhOWaeleTdib3jyTEEIppUnGkyuurLZ9ZCYNydgVQbNPicgTGASbJe/0, createdBy=cbaa2004663, createdName=山丫, createdTime=Tue Oct 03 05:36:43 CST 2017, time=2017-10-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1472568, encodeId=75b414e25681e, content=<a href='/topic/show?id=ba4a6436107' target=_blank style='color:#2F92EE;'>#污染#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64361, encryptionId=ba4a6436107, topicName=污染)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=dfe67217539, createdName=小刀医生, createdTime=Sun Oct 01 12:06:00 CST 2017, time=2017-10-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1580725, encodeId=b9da1580e25af, content=<a href='/topic/show?id=ad07e811375' target=_blank style='color:#2F92EE;'>#细菌污染#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78113, encryptionId=ad07e811375, topicName=细菌污染)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=2bf816641245, createdName=1249894fm78暂无昵称, createdTime=Sun Oct 01 12:06:00 CST 2017, time=2017-10-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=248896, encodeId=7357248896e8, content=好文章.学习了., beContent=null, objectType=article, channel=null, level=null, likeNumber=58, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=60791943465, createdName=1e0f8808m18(暂无匿称), createdTime=Fri Sep 29 21:50:13 CST 2017, time=2017-09-29, status=1, ipAttribution=)]
    2018-02-28 luwei00

    #牙髓病#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1669604, encodeId=3072166960482, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Wed May 02 21:06:00 CST 2018, time=2018-05-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1953612, encodeId=ee681953612d6, content=<a href='/topic/show?id=86156e68526' target=_blank style='color:#2F92EE;'>#牙髓病#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67685, encryptionId=86156e68526, topicName=牙髓病)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e093393, createdName=luwei00, createdTime=Wed Feb 28 10:06:00 CST 2018, time=2018-02-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=249839, encodeId=dd552498392c, content=学习了.担忧, beContent=null, objectType=article, channel=null, level=null, likeNumber=40, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzbCrNlTIlTfsCoY1L9dvM1PibZhOWaeleTdib3jyTEEIppUnGkyuurLZ9ZCYNydgVQbNPicgTGASbJe/0, createdBy=cbaa2004663, createdName=山丫, createdTime=Tue Oct 03 05:36:43 CST 2017, time=2017-10-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1472568, encodeId=75b414e25681e, content=<a href='/topic/show?id=ba4a6436107' target=_blank style='color:#2F92EE;'>#污染#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64361, encryptionId=ba4a6436107, topicName=污染)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=dfe67217539, createdName=小刀医生, createdTime=Sun Oct 01 12:06:00 CST 2017, time=2017-10-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1580725, encodeId=b9da1580e25af, content=<a href='/topic/show?id=ad07e811375' target=_blank style='color:#2F92EE;'>#细菌污染#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78113, encryptionId=ad07e811375, topicName=细菌污染)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=2bf816641245, createdName=1249894fm78暂无昵称, createdTime=Sun Oct 01 12:06:00 CST 2017, time=2017-10-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=248896, encodeId=7357248896e8, content=好文章.学习了., beContent=null, objectType=article, channel=null, level=null, likeNumber=58, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=60791943465, createdName=1e0f8808m18(暂无匿称), createdTime=Fri Sep 29 21:50:13 CST 2017, time=2017-09-29, status=1, ipAttribution=)]
    2017-10-03 山丫

    学习了.担忧

    0

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    2017-10-01 小刀医生

    #污染#

    0

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    2017-10-01 1249894fm78暂无昵称

    #细菌污染#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1669604, encodeId=3072166960482, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Wed May 02 21:06:00 CST 2018, time=2018-05-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1953612, encodeId=ee681953612d6, content=<a href='/topic/show?id=86156e68526' target=_blank style='color:#2F92EE;'>#牙髓病#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67685, encryptionId=86156e68526, topicName=牙髓病)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e093393, createdName=luwei00, createdTime=Wed Feb 28 10:06:00 CST 2018, time=2018-02-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=249839, encodeId=dd552498392c, content=学习了.担忧, beContent=null, objectType=article, channel=null, level=null, likeNumber=40, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzbCrNlTIlTfsCoY1L9dvM1PibZhOWaeleTdib3jyTEEIppUnGkyuurLZ9ZCYNydgVQbNPicgTGASbJe/0, createdBy=cbaa2004663, createdName=山丫, createdTime=Tue Oct 03 05:36:43 CST 2017, time=2017-10-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1472568, encodeId=75b414e25681e, content=<a href='/topic/show?id=ba4a6436107' target=_blank style='color:#2F92EE;'>#污染#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64361, encryptionId=ba4a6436107, topicName=污染)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=dfe67217539, createdName=小刀医生, createdTime=Sun Oct 01 12:06:00 CST 2017, time=2017-10-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1580725, encodeId=b9da1580e25af, content=<a href='/topic/show?id=ad07e811375' target=_blank style='color:#2F92EE;'>#细菌污染#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78113, encryptionId=ad07e811375, topicName=细菌污染)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=2bf816641245, createdName=1249894fm78暂无昵称, createdTime=Sun Oct 01 12:06:00 CST 2017, time=2017-10-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=248896, encodeId=7357248896e8, content=好文章.学习了., beContent=null, objectType=article, channel=null, level=null, likeNumber=58, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=60791943465, createdName=1e0f8808m18(暂无匿称), createdTime=Fri Sep 29 21:50:13 CST 2017, time=2017-09-29, status=1, ipAttribution=)]
    2017-09-29 1e0f8808m18(暂无匿称)

    好文章.学习了.

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