J Endod:磷酸铌玻璃类牙胶尖在体外对牙周膜成纤维细胞的毒性作用

2020-07-27 lishiting MedSci原创

根管封闭的效果可能会受到所选择的充填材料化学成分的影响。这篇研究的目的是在体外评估GNB牙胶尖对人牙周膜成纤维细胞(PDLF)的毒性和细胞调节作用。

根管封闭的效果可能会受到所选择的充填材料化学成分的影响。磷酸铌玻璃类牙胶(GNB)被建议可用作生物材料类的封闭牙尖。这篇研究的目的是在体外评估GNB牙胶尖对人牙周膜成纤维细胞(PDLF)的毒性和细胞调节作用。

研究培养人的PDLF。获取规则的牙胶尖(GP)和GNB浸提物,连续稀释(1:5, 1:10, and 1:25)以用来刺激PDLF。采用Alamar blue reagent检测细胞活力,通过RT-qPCR检测I型胶原蛋白(COL-1)和牙骨质蛋白1 (CEMP-1)的表达。通过one-way analysis of variance和Tukey's post检验进行统计学评估(p < 0.05)。

结果显示,规则的GP仅在纯浸提物时降低细胞活性,而GNB在纯浸提物以及1/5和1/10稀释浓度时展现出对PDLF的毒性。仅在GNB组中的COL-1基因表达下调(p < 0.05)。而2种测试材料的CEMP-1表达未见明显变化。

结论:GP牙胶尖中添加磷酸铌玻璃增加毒性,并影响PDLF活力以及部分干扰细胞的生理学功能。

原始出处:

C C B Meneses, L T Olivi, et al. Cytotoxic effect of niobium phosphate glass-based gutta-percha points on periodontal ligament fibroblasts in vitro. J Endod., 2020 Jun 29;S0099-2399(20)30427-1. doi: 10.1016/j.joen.2020.06.016.

 

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    2020-10-30 xqptu
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