Lasers Med Sci:蓝光发光二极管的照射增强了体外牙龈间充质干细胞的成骨分化

2019-03-04 不详 网络

本研究旨在探究蓝光照射对干细胞成骨分化过程的影响。使用的细胞来自人牙龈间充质干细胞(hGMSC),并使用蓝色发光二极管用0(对照组),1,2,4或6J /cm 2蓝光处理。通过3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四唑溴化物(MTT)细胞增殖试验评估细胞生长,并通过监测碱性磷酸酶(ALP)活性来评估成骨分化,茜素红染色和实时PCR(RT-PCR)。 MTT测定的结果表明蓝光抑制

本研究旨在探究蓝光照射对干细胞成骨分化过程的影响。

使用的细胞来自人牙龈间充质干细胞(hGMSC),并使用蓝色发光二极管用0(对照组),1,2,4或6J /cm 2蓝光处理。通过3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四唑溴化物(MTT)细胞增殖试验评估细胞生长,并通过监测碱性磷酸酶(ALP)活性来评估成骨分化,茜素红染色和实时PCR(RT-PCR)。

MTT测定的结果表明蓝光抑制hGMSC增殖,ALP和茜素红结果显示蓝光促进成骨。当细胞用2或4J /cm 2的蓝光照射时,成骨基因runt相关转录因子2(Runx2),I型胶原(Col1)和骨钙蛋白(OCN)的表达水平显著增加(P <0.05)。

总之,该研究结果表明,蓝光照射抑制hGMSC的增殖并促进成骨分化。

原始出处:

Zhu T, Wu Y, et al., Irradiation by blue light-emitting diode enhances osteogenic differentiation in gingival mesenchymal stem cells in vitro. Lasers Med Sci. 2019 Mar 2. doi: 10.1007/s10103-019-02750-3.

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    2019-06-21 HinsMax
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    2019-12-21 anminleiryan
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    2019-03-06 zhaojie88
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