Clin Chem:宏基因组分析显示临床的SARS-CoV-2感染和细菌或病毒的超感染和定植?

2020-08-01 MedSci原创 MedSci原创

从最初描述SARS-CoV-2到发现其在美国广泛传播相隔了2个多月。尽管间隔时间很长,但以PCR为基础的特异性定量逆转录(qRT) SARS-CoV-2检测在美国的实施一直缓慢,且仍未广泛开展检测。

从最初描述SARS-CoV-2到发现其在美国广泛传播相隔了2个多月。尽管间隔时间很长,但以PCR为基础的特异性定量逆转录(qRT) SARS-CoV-2检测在美国的实施一直缓慢,且仍未广泛开展检测。

宏基因组测序为新出现的病原体的无偏性检测提供了希望,而无需事先了解病原的身份或其基因组序列。为了评估在当前SARS-CoV-2流行背景下的宏基因组方法,对来自华盛顿州西雅图的实验室确认的阳性和阴性样本,通过宏基因组测序进行评估,并与在SARS-CoV-2出现之前创建的2019年参考基因组数据库进行比较。

在实验室验证的SARS-CoV-2病例中,我们在36小时内的结果清楚地鉴定出了一种新的人类Betacoronavirus,与已知的蝙蝠Betacoronavirus密切相关。 一部分样本还显示出人副流感病毒3或莫拉氏菌属的过度感染或定殖,这突出表明需要直接测试SARS-CoV-2,而不是使用病毒性呼吸系统来排除感染。 RT-PCR对SARS-CoV-2阴性的样本经宏基因组分析也阴性,鼻病毒A和C呈阳性。与靶向SARS-CoV-2 qRT-PCR测试不同,这些SARS-CoV-2阴性样本的宏基因组分析 确定了患者呼吸系统症状的候选病因。

综上所述,这些结果证明了宏基因组分析在监测和应对这次和未来的病毒大流行方面的价值。

原始出处:

Vikas Peddu, Ryan C Shean,Metagenomic Analysis Reveals Clinical SARS-CoV-2 Infection and Bacterial or Viral Superinfection and Colonization

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    2021-06-13 hukaixun
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    2020-08-03 小刀医生
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    2020-08-01 gq1688888

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