HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging

Safieddine, A; Coleno, E; Lionneton, F; Traboulsi, AM; Salloum, S; Lecellier, CH; Gostan, T; Georget, V; Hassen-Khodja, C; Imbert, A; Mueller, F; Walter, T; Peter, M; Bertrand, E

Safieddine, A; Bertrand, E (通讯作者),Univ Montpellier, CNRS, Inst Genet Mol Montpellier, Montpellier, France.;Safieddine, A (通讯作者),Sorbonne Univ, CNRS, Inst Biol Paris Seine, Lab Biol Dev, Paris, France.;Bertrand, E (通讯作者),Univ Montpellier, CNRS, Inst Genet Humaine, Montpellier, France.;Bertrand, E (通讯作者),Univ Montpellier, CNRS, Equipe Labelisee Ligue Natl Canc, Montpellier, France.

NATURE PROTOCOLS, 2023; 18 (1): 157

Abstract

The ability to visualize RNA in its native subcellular environment by using single-molecule fluorescence in situ hybridization (smFISH) has reshaped o......

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