ARCH PATHOL LAB MED：多步程序法在皮肤黑色素瘤前哨淋巴结分析中的应用

2019-10-24 MedSci MedSci原创

目前，对于评估前哨淋巴结(SLNs)在皮肤黑色素瘤，还没有通用的检测程序。许多机构使用多步骤的方法处理多个苏木精-伊红(H&E)和免疫组织化学染色。然而，这可能是一项昂贵的、耗费时间和资源的工作。

目前，对于评估前哨淋巴结(SLNs)在皮肤黑色素瘤，还没有通用的检测程序。许多机构使用多步骤的方法处理多个苏木精-伊红(H&E)和免疫组织化学染色。然而，这可能是一项昂贵的、耗费时间和资源的工作。

本研究通过具体评估卡尔加里实验室服务(CLS)方案(包含3张H&E载玻片和1张S100蛋白，1张HMB-45和1张Melan-A载玻片，每个黑色素瘤SLN块)来评估多步骤方案在分析黑色素瘤SLN中的效用，并试图开发更为精简的方案。

CLS皮肤病理组接收来自194例确诊为皮肤黑色素瘤患者的SLN切片的组织学切片。组织块按CLS SLN方案处理。研究人员在显微镜下按照协议的每个步骤对这些玻片重新核对以确定是否转移性黑色素瘤。然后使用SPSS软件创建一个决策树，以确定哪个步骤最准确地反映真实的诊断结果。

研究发现在Melan-A免疫染色中，337个阴性的SLNs中有337个(100%)可被正确诊断为阴性，56个阳性淋巴结中有55个(98.2%)可被正确诊断为阳性。随着H&E水平的增加，393个单核细胞中的393个(100%)可被准确诊断。

本研究建议常规的黑色素瘤SLN评估方案应该仅限于2张玻片，包括1张H&E染色和1张Melan-A染色。该技术方案既省时又经济，诊断准确性也较高。

原始出处：

Pavandeep Gill, MD; Jenika Howell, MD;Utility of Multistep Protocols in the Analysis of Sentinel Lymph Nodes in Cutaneous Melanoma: An Assessment of 194 Cases

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2020-07-24 sunylz

#色素#

22 0
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2020-08-23 yb6560

#Pathol#

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2019-10-26 sodoo

#淋巴结#

29 0
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2019-10-26 zhouqu_8

#皮肤黑色素瘤#

33 0
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2019-10-26 by2013

#前哨淋巴结#

32 0
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2019-10-26 cqlidoudou

#黑色素#

18 0
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2019-10-26 cqlidoudou

#黑色素#

23 0
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2019-10-26 ms3994565386320060

#Med#

17 0

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科研数智化

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皮肤性

ARCH PATHOL LAB MED：多步程序法在皮肤黑色素瘤前哨淋巴结分析中的应用

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