J Pharm Bioallied Sci:聚合酶链反应鉴定有无胃炎患者唾液中的幽门螺杆菌

2020-03-04 不详 网络

本研究旨在通过聚合酶链反应(PCR)方法鉴定有和无胃炎的患者唾液中是否存在幽门螺杆菌。该研究纳入第一组中有胃炎各种症状的20例患者和第二组中的10例无症状受试者。从20例有胃十二指肠疾病的有症状患者中收集肠道内窥镜肛门活检。在内窥镜检查之前从所有患者中获取唾液标本。使用从患者的唾液和活检物中分离的基因组DNA作为模板进行PCR,以检测幽门螺杆菌中16S核糖体RNA基因的存在。结果显示,在第一组中,

本研究旨在通过聚合酶链反应(PCR)方法鉴定有和无炎的患者唾液中是否存在幽门螺杆菌。

该研究纳入第一组中有炎各种症状的20例患者和第二组中的10例无症状受试者。从20例有胃十二指肠疾病的有症状患者中收集肠道内窥镜肛门活检。在内窥镜检查之前从所有患者中获取唾液标本。使用从患者的唾液和活检物中分离的基因组DNA作为模板进行PCR,以检测幽门螺杆菌中16S核糖体RNA基因的存在。

结果显示,在第一组中,有10例(50%)临床胃炎通过内窥镜活检对幽门螺杆菌呈阳性,而10例(50%)阴性。在10例幽门螺杆菌内镜活检阳性病例中,唾液中PCR阳性8例,阴性2例。在10例内镜活检阴性病例中,3例唾液中幽门螺杆菌PCR阳性,7例阴性。在第二组中,有四个是胃炎的症状,六个是阴性。在六例胃炎阴性病例中,三例为PCR阳性,四例为胃炎阳性,三例为PCR阳性。发现PCR的敏感性和特异性分别为80%和70%。唾液中PCR的阳性预测值和阴性预测值分别为72.7%和77.7%。

总之,该研究结果表明,唾液的PCR分析可能有助于识别幽门螺杆菌,并作为诊断和监测预后的非创伤性技术。

原始出处:

E. V. Soma Sekhar Goud, Ranganathan Kannan, et al., Identification of Helicobacter pylori in Saliva of Patients with and without Gastritis by Polymerase Chain Reaction. J Pharm Bioallied Sci. 2019 Nov; 11(Suppl 3): S523–S529.

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    2020-05-19 kcb069
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    2020-05-09 sunylz
  5. [GetPortalCommentsPageByObjectIdResponse(id=1811817, encodeId=5dc8181181e3a, content=<a href='/topic/show?id=751f8889423' target=_blank style='color:#2F92EE;'>#螺杆菌#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=88894, encryptionId=751f8889423, topicName=螺杆菌)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=87b1109, createdName=kcb069, createdTime=Tue May 19 15:47:00 CST 2020, time=2020-05-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1787650, encodeId=5bd91e87650e6, content=<a href='/topic/show?id=fa03141591e' target=_blank style='color:#2F92EE;'>#Pharm#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14159, encryptionId=fa03141591e, topicName=Pharm)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=778d36, createdName=柳叶一刀, createdTime=Sat Dec 26 03:47:00 CST 2020, time=2020-12-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1807758, encodeId=8afe180e75814, content=<a href='/topic/show?id=c3198081280' target=_blank style='color:#2F92EE;'>#聚合酶链反应#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80812, encryptionId=c3198081280, topicName=聚合酶链反应)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1376173, createdName=linagood, createdTime=Tue Dec 08 23:47:00 CST 2020, time=2020-12-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1919004, encodeId=c4c219190044d, content=<a href='/topic/show?id=4f26341994' target=_blank style='color:#2F92EE;'>#Bio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3419, encryptionId=4f26341994, topicName=Bio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=12de429, createdName=sunylz, createdTime=Sat May 09 22:47:00 CST 2020, time=2020-05-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1955991, encodeId=1dd919559910a, content=<a href='/topic/show?id=30ac80810c0' target=_blank style='color:#2F92EE;'>#聚合酶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80810, encryptionId=30ac80810c0, topicName=聚合酶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a5712500001, createdName=晓辰, createdTime=Fri Jun 12 20:47:00 CST 2020, time=2020-06-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1329347, encodeId=8c75132934e71, content=<a href='/topic/show?id=bb96232801' target=_blank style='color:#2F92EE;'>#ALL#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2328, encryptionId=bb96232801, topicName=ALL)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdqq.qlogo.cn/qqapp/1103841572/C2EFD5CFB07FE291138AD4FD0C98E3DE/100, createdBy=e3d52500031, createdName=膀胱癌, createdTime=Thu Mar 05 16:47:00 CST 2020, time=2020-03-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=380052, encodeId=50f538005255, content=这个有这意思,可以看看和胃的关系。, beContent=null, objectType=article, channel=null, level=null, likeNumber=53, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=43a21971117, createdName=njwbhuang, createdTime=Wed Mar 04 06:30:13 CST 2020, time=2020-03-04, status=1, ipAttribution=)]
    2020-06-12 晓辰
  6. [GetPortalCommentsPageByObjectIdResponse(id=1811817, encodeId=5dc8181181e3a, content=<a href='/topic/show?id=751f8889423' target=_blank style='color:#2F92EE;'>#螺杆菌#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=88894, encryptionId=751f8889423, topicName=螺杆菌)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=87b1109, createdName=kcb069, createdTime=Tue May 19 15:47:00 CST 2020, time=2020-05-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1787650, encodeId=5bd91e87650e6, content=<a href='/topic/show?id=fa03141591e' target=_blank style='color:#2F92EE;'>#Pharm#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14159, encryptionId=fa03141591e, topicName=Pharm)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=778d36, createdName=柳叶一刀, createdTime=Sat Dec 26 03:47:00 CST 2020, time=2020-12-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1807758, encodeId=8afe180e75814, content=<a href='/topic/show?id=c3198081280' target=_blank style='color:#2F92EE;'>#聚合酶链反应#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80812, encryptionId=c3198081280, topicName=聚合酶链反应)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1376173, createdName=linagood, createdTime=Tue Dec 08 23:47:00 CST 2020, time=2020-12-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1919004, encodeId=c4c219190044d, content=<a href='/topic/show?id=4f26341994' target=_blank style='color:#2F92EE;'>#Bio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3419, encryptionId=4f26341994, topicName=Bio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=12de429, createdName=sunylz, createdTime=Sat May 09 22:47:00 CST 2020, time=2020-05-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1955991, encodeId=1dd919559910a, content=<a href='/topic/show?id=30ac80810c0' target=_blank style='color:#2F92EE;'>#聚合酶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80810, encryptionId=30ac80810c0, topicName=聚合酶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a5712500001, createdName=晓辰, createdTime=Fri Jun 12 20:47:00 CST 2020, time=2020-06-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1329347, encodeId=8c75132934e71, content=<a href='/topic/show?id=bb96232801' target=_blank style='color:#2F92EE;'>#ALL#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2328, encryptionId=bb96232801, topicName=ALL)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdqq.qlogo.cn/qqapp/1103841572/C2EFD5CFB07FE291138AD4FD0C98E3DE/100, createdBy=e3d52500031, createdName=膀胱癌, createdTime=Thu Mar 05 16:47:00 CST 2020, time=2020-03-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=380052, encodeId=50f538005255, content=这个有这意思,可以看看和胃的关系。, beContent=null, objectType=article, channel=null, level=null, likeNumber=53, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=43a21971117, createdName=njwbhuang, createdTime=Wed Mar 04 06:30:13 CST 2020, time=2020-03-04, status=1, ipAttribution=)]
    2020-03-05 膀胱癌
  7. [GetPortalCommentsPageByObjectIdResponse(id=1811817, encodeId=5dc8181181e3a, content=<a href='/topic/show?id=751f8889423' target=_blank style='color:#2F92EE;'>#螺杆菌#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=88894, encryptionId=751f8889423, topicName=螺杆菌)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=87b1109, createdName=kcb069, createdTime=Tue May 19 15:47:00 CST 2020, time=2020-05-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1787650, encodeId=5bd91e87650e6, content=<a href='/topic/show?id=fa03141591e' target=_blank style='color:#2F92EE;'>#Pharm#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14159, encryptionId=fa03141591e, topicName=Pharm)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=778d36, createdName=柳叶一刀, createdTime=Sat Dec 26 03:47:00 CST 2020, time=2020-12-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1807758, encodeId=8afe180e75814, content=<a href='/topic/show?id=c3198081280' target=_blank style='color:#2F92EE;'>#聚合酶链反应#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80812, encryptionId=c3198081280, topicName=聚合酶链反应)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1376173, createdName=linagood, createdTime=Tue Dec 08 23:47:00 CST 2020, time=2020-12-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1919004, encodeId=c4c219190044d, content=<a href='/topic/show?id=4f26341994' target=_blank style='color:#2F92EE;'>#Bio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3419, encryptionId=4f26341994, topicName=Bio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=12de429, createdName=sunylz, createdTime=Sat May 09 22:47:00 CST 2020, time=2020-05-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1955991, encodeId=1dd919559910a, content=<a href='/topic/show?id=30ac80810c0' target=_blank style='color:#2F92EE;'>#聚合酶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=80810, encryptionId=30ac80810c0, topicName=聚合酶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a5712500001, createdName=晓辰, createdTime=Fri Jun 12 20:47:00 CST 2020, time=2020-06-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1329347, encodeId=8c75132934e71, content=<a href='/topic/show?id=bb96232801' target=_blank style='color:#2F92EE;'>#ALL#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=2328, encryptionId=bb96232801, topicName=ALL)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdqq.qlogo.cn/qqapp/1103841572/C2EFD5CFB07FE291138AD4FD0C98E3DE/100, createdBy=e3d52500031, createdName=膀胱癌, createdTime=Thu Mar 05 16:47:00 CST 2020, time=2020-03-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=380052, encodeId=50f538005255, content=这个有这意思,可以看看和胃的关系。, beContent=null, objectType=article, channel=null, level=null, likeNumber=53, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=43a21971117, createdName=njwbhuang, createdTime=Wed Mar 04 06:30:13 CST 2020, time=2020-03-04, status=1, ipAttribution=)]
    2020-03-04 njwbhuang

    这个有这意思,可以看看和胃的关系。

    0