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Sci Rep：GO-PLGA/HA微载体固定BMP-2可促进骨整合的发生

2017-10-26 MedSci MedSci原创

GO-PLGA/HABMP-2骨整合
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缺乏生物活性严重限制了可降解骨组织工程生物植体的开发。因此，对复合材料的表面改性成了改善骨再生中骨整合的关键。骨形态发生蛋白-2（BMP-2）是诱导骨形成和促进骨再生的关键因子，目前已广泛应用于各种临床治疗试验。本研究中，研究人员成功地将BMP-2固定在掺入氧化石墨烯的PLGA/HA（GO-PLGA / HA）可生物降解微载体上。研究结果表明，氧化石墨烯（GO）在120分钟内可简单且高效促进肽在P

缺乏生物活性严重限制了可降解骨组织工程生物植体的开发。因此，对复合材料的表面改性成了改善骨再生中骨整合的关键。骨形态发生蛋白-2（BMP-2）是诱导骨形成和促进骨再生的关键因子，目前已广泛应用于各种临床治疗试验。

本研究中，研究人员成功地将BMP-2固定在掺入氧化石墨烯的PLGA/HA（GO-PLGA / HA）可生物降解微载体上。研究结果表明，氧化石墨烯（GO）在120分钟内可简单且高效促进肽在PLGA/HA微载体上的固定。为了行进一步的体外测试，研究人员将MC3T3-E1细胞在不同的微载体上培养以观察各种细胞的活性。结果显示，GO和HA可明显增强细胞的粘附和增殖。更重要的是，BMP-2在GO-PLGA/HA微载体上的固定化使得体外细胞成骨分化明显更高，碱性磷酸酶活性试验、定量实时聚合酶链反应分析、免疫荧光染色和基质矿化检测证实了这一点。

总之，本研究的结果表明，使用GO-PLGA/HA微载体固定BMP-2的方法对于增强骨植体的骨整合具有很大的潜力。

原始出处：

Chuan Fu, Xiaoyu Yang, et al., Enhancing Cell Proliferation and Osteogenic Differentiation of MC3T3-E1 Pre-osteoblasts by BMP-2 Delivery in Graphene Oxide-Incorporated PLGA/HA Biodegradable Microcarriers. Sci Rep. 2017; 7: 12549. Published online 2017 Oct 2. doi:  10.1038/s41598-017-12935-x


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    2018-08-11 12498f17m73暂无昵称

    #BMP-2#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1642642, encodeId=273b164264208, content=<a href='/topic/show?id=814c35e6e2' target=_blank style='color:#2F92EE;'>#BMP-2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=48, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3576, encryptionId=814c35e6e2, topicName=BMP-2)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=120c22999408, createdName=12498f17m73暂无昵称, createdTime=Sat Aug 11 12:26:00 CST 2018, time=2018-08-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1704793, encodeId=e3821e0479343, content=<a href='/topic/show?id=0d28143e392' target=_blank style='color:#2F92EE;'>#PLGA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14373, encryptionId=0d28143e392, topicName=PLGA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c02c30837939, createdName=venlin, createdTime=Sun Sep 09 20:26:00 CST 2018, time=2018-09-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1634009, encodeId=81bd163400925, content=<a href='/topic/show?id=596635e549' target=_blank style='color:#2F92EE;'>#BMP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3575, encryptionId=596635e549, topicName=BMP)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d95322064897, createdName=HNYYM, createdTime=Sat Sep 01 01:26:00 CST 2018, time=2018-09-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1645441, encodeId=554e1645441b8, content=<a href='/topic/show?id=db329364360' target=_blank style='color:#2F92EE;'>#载体#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=93643, encryptionId=db329364360, topicName=载体)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=58cb23213384, createdName=mei539, createdTime=Thu Aug 02 17:26:00 CST 2018, time=2018-08-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1521744, encodeId=9e881521e44d4, content=<a href='/topic/show?id=0b8f101e026a' target=_blank style='color:#2F92EE;'>#骨整合#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101702, encryptionId=0b8f101e026a, topicName=骨整合)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=03f111392267, createdName=bnurmamat, createdTime=Sat Oct 28 11:26:00 CST 2017, time=2017-10-28, status=1, ipAttribution=)]
    2018-09-09 venlin

    #PLGA#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1642642, encodeId=273b164264208, content=<a href='/topic/show?id=814c35e6e2' target=_blank style='color:#2F92EE;'>#BMP-2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=48, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3576, encryptionId=814c35e6e2, topicName=BMP-2)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=120c22999408, createdName=12498f17m73暂无昵称, createdTime=Sat Aug 11 12:26:00 CST 2018, time=2018-08-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1704793, encodeId=e3821e0479343, content=<a href='/topic/show?id=0d28143e392' target=_blank style='color:#2F92EE;'>#PLGA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14373, encryptionId=0d28143e392, topicName=PLGA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c02c30837939, createdName=venlin, createdTime=Sun Sep 09 20:26:00 CST 2018, time=2018-09-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1634009, encodeId=81bd163400925, content=<a href='/topic/show?id=596635e549' target=_blank style='color:#2F92EE;'>#BMP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3575, encryptionId=596635e549, topicName=BMP)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d95322064897, createdName=HNYYM, createdTime=Sat Sep 01 01:26:00 CST 2018, time=2018-09-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1645441, encodeId=554e1645441b8, content=<a href='/topic/show?id=db329364360' target=_blank style='color:#2F92EE;'>#载体#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=93643, encryptionId=db329364360, topicName=载体)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=58cb23213384, createdName=mei539, createdTime=Thu Aug 02 17:26:00 CST 2018, time=2018-08-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1521744, encodeId=9e881521e44d4, content=<a href='/topic/show?id=0b8f101e026a' target=_blank style='color:#2F92EE;'>#骨整合#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101702, encryptionId=0b8f101e026a, topicName=骨整合)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=03f111392267, createdName=bnurmamat, createdTime=Sat Oct 28 11:26:00 CST 2017, time=2017-10-28, status=1, ipAttribution=)]
    2018-09-01 HNYYM

    #BMP#

    0

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    2018-08-02 mei539

    #载体#

    0

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    2017-10-28 bnurmamat

    #骨整合#

    0