高通量宏基因组测序技术检测病原微生物的临床应用规范化专家共识

2020-12-30 中华医学会检验医学分会 中华检验医学杂志.2020.43(12):1181-1195.

宏基因组下一代测序(mNGS)技术直接针对标本中核酸无偏倚检测病原微生物序列。但是,mNGS需经标本前处理、核酸提取、文库制备、上机测序、数据库比对、报告生成及结果解读等一系列过程,对技术平台及人员素

中文标题:

高通量宏基因组测序技术检测病原微生物的临床应用规范化专家共识

发布日期:

2020-12-30

简要介绍:

宏基因组下一代测序(mNGS)技术直接针对标本中核酸无偏倚检测病原微生物序列。但是,mNGS需经标本前处理、核酸提取、文库制备、上机测序、数据库比对、报告生成及结果解读等一系列过程,对技术平台及人员素质要求较高。为规范mNGS技术在感染性疾病诊断中的应用,提高危急重症、疑难感染性疾病和新发突发传染病的救治水平,在多项国家科技专项的支持下,本领域有关专家起草了本共识,以促进mNGS技术的规范应用和良性发展。

 

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#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=1198099, encodeId=50141198099be, content=实用 学习了, beContent=null, objectType=guider, channel=null, level=null, likeNumber=46, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0c7f5291462, createdName=148645bdm06暂无昵称, createdTime=Mon Feb 28 18:05:29 CST 2022, time=2022-02-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1073577, encodeId=ded210e357733, content=实用,具有指导意义, beContent=null, objectType=guider, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIibkeib472Acn2YjahFJtH2rfrLrBZLCDsZkshGJhQE1Dib3muxRXlmx7olu8QIw2T0t4c4o7rcWJ5Q/132, createdBy=47672297910, createdName=hyf1971, createdTime=Wed Nov 24 18:00:02 CST 2021, time=2021-11-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1067270, encodeId=e4bc106e27028, content=NGS是微生物鉴定的前沿技术应用越来越多,这个规范有助于我们对报告的解读。, beContent=null, objectType=guider, channel=null, level=null, likeNumber=48, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/KWhSPGbJlOTjdyUnSckyLu3BI5gQN0GIIX1Aob1B1Ijcqz86r3bg7kmAjAmfXJbUryR2ehK28gFiaC7CWlcBiahg/132, createdBy=327b5320277, createdName=clj, createdTime=Fri Nov 05 13:06:16 CST 2021, time=2021-11-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=967506, encodeId=236396e5062f, content=同意, beContent=null, objectType=guider, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=90d65504882, createdName=ms5000000620536461, createdTime=Fri May 21 16:07:47 CST 2021, time=2021-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=925469, encodeId=dfda9254693f, content=新技术的解读必需规范, beContent=null, objectType=guider, channel=null, level=null, likeNumber=83, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e2ae5459879, createdName=ms6000000631949918, createdTime=Fri Feb 19 14:40:44 CST 2021, time=2021-02-19, status=1, ipAttribution=)]
    2022-02-28 148645bdm06暂无昵称

    实用 学习了

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1198099, encodeId=50141198099be, content=实用 学习了, beContent=null, objectType=guider, channel=null, level=null, likeNumber=46, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0c7f5291462, createdName=148645bdm06暂无昵称, createdTime=Mon Feb 28 18:05:29 CST 2022, time=2022-02-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1073577, encodeId=ded210e357733, content=实用,具有指导意义, beContent=null, objectType=guider, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIibkeib472Acn2YjahFJtH2rfrLrBZLCDsZkshGJhQE1Dib3muxRXlmx7olu8QIw2T0t4c4o7rcWJ5Q/132, createdBy=47672297910, createdName=hyf1971, createdTime=Wed Nov 24 18:00:02 CST 2021, time=2021-11-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1067270, encodeId=e4bc106e27028, content=NGS是微生物鉴定的前沿技术应用越来越多,这个规范有助于我们对报告的解读。, beContent=null, objectType=guider, channel=null, level=null, likeNumber=48, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/KWhSPGbJlOTjdyUnSckyLu3BI5gQN0GIIX1Aob1B1Ijcqz86r3bg7kmAjAmfXJbUryR2ehK28gFiaC7CWlcBiahg/132, createdBy=327b5320277, createdName=clj, createdTime=Fri Nov 05 13:06:16 CST 2021, time=2021-11-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=967506, encodeId=236396e5062f, content=同意, beContent=null, objectType=guider, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=90d65504882, createdName=ms5000000620536461, createdTime=Fri May 21 16:07:47 CST 2021, time=2021-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=925469, encodeId=dfda9254693f, content=新技术的解读必需规范, beContent=null, objectType=guider, channel=null, level=null, likeNumber=83, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e2ae5459879, createdName=ms6000000631949918, createdTime=Fri Feb 19 14:40:44 CST 2021, time=2021-02-19, status=1, ipAttribution=)]
    2021-11-24 hyf1971

    实用,具有指导意义

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1198099, encodeId=50141198099be, content=实用 学习了, beContent=null, objectType=guider, channel=null, level=null, likeNumber=46, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0c7f5291462, createdName=148645bdm06暂无昵称, createdTime=Mon Feb 28 18:05:29 CST 2022, time=2022-02-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1073577, encodeId=ded210e357733, content=实用,具有指导意义, beContent=null, objectType=guider, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIibkeib472Acn2YjahFJtH2rfrLrBZLCDsZkshGJhQE1Dib3muxRXlmx7olu8QIw2T0t4c4o7rcWJ5Q/132, createdBy=47672297910, createdName=hyf1971, createdTime=Wed Nov 24 18:00:02 CST 2021, time=2021-11-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1067270, encodeId=e4bc106e27028, content=NGS是微生物鉴定的前沿技术应用越来越多,这个规范有助于我们对报告的解读。, beContent=null, objectType=guider, channel=null, level=null, likeNumber=48, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/KWhSPGbJlOTjdyUnSckyLu3BI5gQN0GIIX1Aob1B1Ijcqz86r3bg7kmAjAmfXJbUryR2ehK28gFiaC7CWlcBiahg/132, createdBy=327b5320277, createdName=clj, createdTime=Fri Nov 05 13:06:16 CST 2021, time=2021-11-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=967506, encodeId=236396e5062f, content=同意, beContent=null, objectType=guider, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=90d65504882, createdName=ms5000000620536461, createdTime=Fri May 21 16:07:47 CST 2021, time=2021-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=925469, encodeId=dfda9254693f, content=新技术的解读必需规范, beContent=null, objectType=guider, channel=null, level=null, likeNumber=83, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e2ae5459879, createdName=ms6000000631949918, createdTime=Fri Feb 19 14:40:44 CST 2021, time=2021-02-19, status=1, ipAttribution=)]
    2021-11-05 clj

    NGS是微生物鉴定的前沿技术应用越来越多,这个规范有助于我们对报告的解读。

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1198099, encodeId=50141198099be, content=实用 学习了, beContent=null, objectType=guider, channel=null, level=null, likeNumber=46, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0c7f5291462, createdName=148645bdm06暂无昵称, createdTime=Mon Feb 28 18:05:29 CST 2022, time=2022-02-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1073577, encodeId=ded210e357733, content=实用,具有指导意义, beContent=null, objectType=guider, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIibkeib472Acn2YjahFJtH2rfrLrBZLCDsZkshGJhQE1Dib3muxRXlmx7olu8QIw2T0t4c4o7rcWJ5Q/132, createdBy=47672297910, createdName=hyf1971, createdTime=Wed Nov 24 18:00:02 CST 2021, time=2021-11-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1067270, encodeId=e4bc106e27028, content=NGS是微生物鉴定的前沿技术应用越来越多,这个规范有助于我们对报告的解读。, beContent=null, objectType=guider, channel=null, level=null, likeNumber=48, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/KWhSPGbJlOTjdyUnSckyLu3BI5gQN0GIIX1Aob1B1Ijcqz86r3bg7kmAjAmfXJbUryR2ehK28gFiaC7CWlcBiahg/132, createdBy=327b5320277, createdName=clj, createdTime=Fri Nov 05 13:06:16 CST 2021, time=2021-11-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=967506, encodeId=236396e5062f, content=同意, beContent=null, objectType=guider, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=90d65504882, createdName=ms5000000620536461, createdTime=Fri May 21 16:07:47 CST 2021, time=2021-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=925469, encodeId=dfda9254693f, content=新技术的解读必需规范, beContent=null, objectType=guider, channel=null, level=null, likeNumber=83, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e2ae5459879, createdName=ms6000000631949918, createdTime=Fri Feb 19 14:40:44 CST 2021, time=2021-02-19, status=1, ipAttribution=)]
    2021-05-21 ms5000000620536461

    同意

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1198099, encodeId=50141198099be, content=实用 学习了, beContent=null, objectType=guider, channel=null, level=null, likeNumber=46, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0c7f5291462, createdName=148645bdm06暂无昵称, createdTime=Mon Feb 28 18:05:29 CST 2022, time=2022-02-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1073577, encodeId=ded210e357733, content=实用,具有指导意义, beContent=null, objectType=guider, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTIibkeib472Acn2YjahFJtH2rfrLrBZLCDsZkshGJhQE1Dib3muxRXlmx7olu8QIw2T0t4c4o7rcWJ5Q/132, createdBy=47672297910, createdName=hyf1971, createdTime=Wed Nov 24 18:00:02 CST 2021, time=2021-11-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1067270, encodeId=e4bc106e27028, content=NGS是微生物鉴定的前沿技术应用越来越多,这个规范有助于我们对报告的解读。, beContent=null, objectType=guider, channel=null, level=null, likeNumber=48, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/KWhSPGbJlOTjdyUnSckyLu3BI5gQN0GIIX1Aob1B1Ijcqz86r3bg7kmAjAmfXJbUryR2ehK28gFiaC7CWlcBiahg/132, createdBy=327b5320277, createdName=clj, createdTime=Fri Nov 05 13:06:16 CST 2021, time=2021-11-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=967506, encodeId=236396e5062f, content=同意, beContent=null, objectType=guider, channel=null, level=null, likeNumber=59, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=90d65504882, createdName=ms5000000620536461, createdTime=Fri May 21 16:07:47 CST 2021, time=2021-05-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=925469, encodeId=dfda9254693f, content=新技术的解读必需规范, beContent=null, objectType=guider, channel=null, level=null, likeNumber=83, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e2ae5459879, createdName=ms6000000631949918, createdTime=Fri Feb 19 14:40:44 CST 2021, time=2021-02-19, status=1, ipAttribution=)]
    2021-02-19 ms6000000631949918

    新技术的解读必需规范

    0

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