笔记详情
标题
Curcumin Modulates DNA Methylation in Colorectal Cancer Cells
内容
Quantitative Methylation Analyses
We validated methylation microarray data by independent single gene/promoter quantitative methylation assays. Depending on the methylation assay design features, we used either bisulfite pyrosequencing (PSQ HS 96A pyrosequencing system, Qiagen, Valencia, CA) or real-time based qMSP for quantitative methylation analyses as described previously [37], [38]. Primer sequences used for both methods are provided in Supplementary Table S1.
来源
PLoS One. 2013; 8(2): e57709.
类别
关键词
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