笔记详情
标题
drosophila sl2 cell rnai.
内容
Drosophila SL2 cell RNAi.
All RNAi for Drosophila SL2 cells was performed as described [52]. Double-stranded RNA was synthesized with T7 RiboMax (Promega, http://www.promega.com). Cells were RNAi depleted using 50 μg of dsRNA for each gene and normalized with luciferase dsRNA for co-RNAi treatments. For RNAi-transfection experiments, 15 μg of dsRNA was included in each transfection after the initial RNAi depletion. Depletion of RBF1 was confirmed by Western analysis (unpublished data). Depletion of Aac11 RNA was confirmed on microarray analysis (unpublished data).
点击翻译来源
plos genet. 2006 november 2(11): e196
类别
领域
cell biology
关键词
